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Sequencing – Available Platforms

Sequencing Platform Cycles (bp) Number of Reads
*paired-end
Illumina® MiSeq 50, 300, 500 ~30 million for V2 ~50 million for V3
Illumina® NextSeq 500 MO 75, 150, 300 ~260 million
Illumina® NextSeq 500 HO 75, 150, 300 ~400 million
Illumina® NovaSeq 6000 SP 100, 200, 300 ~1.6 billion  
Illumina® NovaSeq 6000 S1 100, 200, 300 ~3.2 billion
Illumina® NovaSeq 6000 S2 100, 200, 300 ~8.2 billion
Illumina® NovaSeq 6000 S4 35, 200, 300 ~20 billion
Illumina® NovaSeq X Plus 1.5B 100, 200, 300 ~3 billion
Illumina® NovaSeq X Plus 10B 100, 200, 300 ~20 billion
Illumina® NovaSeq X Plus 25B 300 ~50 billion
Element AVITI™ Cloudbreak Freestyle Low Output 300 ~250 million
Element AVITI™ Cloudbreak Freestyle Mid Output 150, 300, 600 ~500 million *600 cycle kit ~100 million
Element AVITI™ Cloudbreak Freestyle High Output 150, 300, 600 ~1 billion *600 cycle kit ~300 million
Long-Read Sequencing Platform Flowcell Minimum Output (Gb)
PacBio Revio™ 1 SMRTcell
(Run Time: 12, 24, or 30hrs)
~60-90
Oxford Nanopore Technologies® 1 flowcell ~50-150