Pre-GEM
Check nuclear morphology for high-quality nuclei.
Post-cDNA Amplification (for Gene Expression Library Prep Only)
An aliquot is run on the Bioanalyzer to calculate the amount of cDNA to move into the 3′ Gene Expression Library Construction step.
Post-Library Prep
Final libraries are run on qPCR and the Bioanalyzer to gather concentration and insert size information, respectively.