Chromium Next GEM Single Cell Multiome ATAC + Gene Expression Library Prep Post-GEM Generation
ATAC Library Construction
P7 and a sample index are added to pre-amplified transposed DNA during ATAC library construction via PCR. The final ATAC libraries contain the P5 and P7 sequences used in Illumina® bridge amplification.
Gene Expression Library Construction
Enzymatic fragmentation and size selection are used to optimize the cDNA amplicon size. P5, P7, i7 and i5 sample indexes, and TruSeq Read 2 (read 2 primer sequence) are added via End Repair, A-tailing, Adaptor Ligation, and PCR. The final gene expression libraries contain the P5 and P7 primers used in Illumina® bridge amplification.
